Microfluidics for Infectious Disease Applications (microbial and viral pathogens)
Microfluidic systems, particular droplet-based microfluidic systems, are ideal for single-cell resolution studies and cell phenotyping/screening assays at extremely high throughput. Our lab is developing integrated microfluidic systems for microbial pathogen studies, developing therapeutics and countermeasure against infectious diseases (both microbial and viral pathogens), as well as for conducting fundamental host-pathogen interaction studies.
Topics
Bacterial Pathogen Analyses and Phenotyping
We are developing continuous-flow microfluidics and droplet microfluidic systems enabling rapid single-cell-resolution bacterial pathogenicity profiling.
Selected Project/Publications
- Phenotype-Based Threat Assessment
J. Yang, M. Eslami, Y. Chen, M. Das, D. Zhang, S. Chen, A. Roberts, M. Weston, A. Volkova, K. Faghihi, R. K. Moore, R. C. Alaniz, A. R. Wattam, A. Dickerman, C. Cucinell, J. Kendziorski, S. Coburn, H. Paterson, O. Obanor, J. Maples, S. Servetas, J. Dootz, Q. -M. Qin, J. E. Samuel*, A. Han*, E. J. van Schaik*, and P. de Figueiredo* (*co-corresponding authors),
Proceedings of the National Academy of Sciences, Vol. 119 (14), e2112886119 (2022) - Development of a Real-Time Microchip PCR System for Portable Plant Disease Diagnosis
C. Koo, M. Malapi-Wight, H. S. Kim, O. S. Cifci, V. L. Vaughn-Diaz, B. Ma, S. Kim, H. Abdel-Raziq, K. Ong, Y. -K. Jo, D. C. Gross, W. -B. Shim, and A. Han,
PLoS ONE, Vol. 8 (12), e82704, (2013)
Understanding Host-Pathogen Interactions
Providing single-cell resolution microfluidic platform for studying how microbial pathogens interact with host cells and impact host cells.
Selected Project/Publications
- An automated system for interrogating the evolution of microbial endosymbiosis
Can Huang, Fengguang Guo, Han Wang, Jasmine Olivares, James Dalton III, Olga Belyanina, Alice R Wattam, Clark A Cucinell, Allan W Dickerman, Qing-Ming Qin, Arum Han, and Paul de Figueiredo,
Lab on a Chip, Vol. 23 (4), pp. 671-683 (2023) - Development of Single-Cell-Level Microfluidic Technology for Long-Term Growth Visualization of Living Cultures of Mycobacterium smegmatis
H. Wang*, G. M. Conover*, S. -I. Han, J. C. Sacchettini, and A. Han (*these authors contributed equally),
Microsystems and Nanoengineering, Vol. 7, Article Number: 37 (2021)
The article was also featured as a cover image
Rapid Countermeasure Development
We are developing high-throughput microfluidic screening platforms to assist rapid countermeasure development against bacterial and viral pathogens.
Selected Project/Publications
- Sub-Second Heat Inactivation of Coronavirus using a Beta-Coronavirus Model
Y. Jiang*, H. Zhang*, J. A. Wippold, J. Gupta, J. Dai, P. de Figueiredo, J. L. Leibowitz, and A. Han(*these authors contributed equally),
Biotechnology and Bioengineering, Vol. 118 (5), pp. 2067-2075 (2021)
Selected as Cover Image (click here for cover image) - PRESCIENT: Platform for the Rapid Evaluation of Antibody Success using Integrated Microfluidics Enabled Technology
J. A. Wippold, H. Wang, J. Tingling, J. L. Leibowitz, P. de Figueiredo, and A. Han,
Lab on a Chip, Vol. 20, pp. 1628-1638 (2020) - A Programmable Microfluidic Cell Array for Combinatorial Drug Screening
J. Kim, D. Taylor, N. Agrawal, H. Kim, H. Wang, A. Han, K. Rege, and A. Jayaraman,
Lab on a Chip, Vol. 12, 1813-1822 (2012)