High-throughput Single-cell-resolution Microfluidics Technology Development
We have been developing various single-cell manipulation technologies in the format of both free-flow and droplet-based microfluidic system. Whether it is a detection method, such as fluorescence, Raman spectroscopy, electrical impedance; or a manipulation method, such as acoustophoresis, dielectrophoresis, or magnetophoresis; we have made many fundamental technological advancements that have the potential to be broadly utilized in many different microfluidic lab-on-a-chip applications.
Topics
Single-cell Manipulation Technologies
Developing single-cell manipulation technologies for on-demand controls in microfluidic systems
Selected Project/Publications
- Acoustofluidic Microdevice for Precise Control of Pressure Nodal Positions
S. Yigit, H. Wang, S. -I. Han, Y. H. Cho, and A. Han,
Microfluidics and Nanofluidics, Vol. 24, 52 (2020) - Separation, Characterization, and Handling of Microalgae by Dielectrophoresis
V. Abt, F. Gringel, A. Han, P. Neubauer, and M. Birkholz,
Microorganisms,8(4), 540 (2020) - Raman Spectroscopy Compatible PDMS Droplet Microfluidic Culture and Analysis Platform towards On-Chip Lipidomics
H. S. Kim*, S. C. Waqued*, D. T. Nodurft, T. P. Devarenne, V. V. Yakovlev, and A. Han (*these authors contributed equally),
Analyst, Vol. 142, pp. 1054-1060 (2017)
Single-cell Sensing Technologies
Developing novel sensing and detection technologies that can be integrated into microfluidic lab-on-a-chip systems
Selected Project/Publications
- Measurement of Dielectric Properties of Cells at Single-Cell Resolution Using Electrorotation
Y. Li, C. Huang, S. -I. Han, and A. Han,
Biomedical Microdevices, Vol. 24, 23 (2022) - High-Throughput and Label-Free Multi-Outlet Cell Counting Using a Single Pair of Impedance Electrodes
N. Sobahi and A. Han,
Biosensors and Bioelectronics, Vol. 166, 12458 (2020) - Impedance Spectroscopy-based Cell/Particle Position Detection in Microfluidic Systems
H. Wang*, N. Sobahi*, and A. Han (*these authors contributed equally),
Lab on a Chip, Vol. 17, pp. 1264-269 (2017) - Microfluidic Acoustophoretic Force Based Low Concentration Oil Separation and Detection from Environment
H.Wang, Z.Liu, C. Koo, S. Kim, Y. Cho, D. -Y. Jang, Y. -J. Kim, and A. Han,
Lab on a Chip, Vol. 14, pp. 947-956 (2014)
Microdroplet Manipulation Technologies
Advancing droplet microfluidic manipulation technologies, especially focusing on high efficiency, low error, and integrability.
Selected Project/Publications
- FIDELITY: A quality control system for droplet microfluidics
Han Zhang, Can Huang, Yuwen Li, Rohit Gupte, Ryan Samuel, Jing Dai, Adrian Guzman, Rushant Sabnis, Paul de Figueiredo, Arum Han,
Science Advances, Vol. 8 (27), pp. eabc9108 (2022) - Cell Washing and Solution Exchange in Droplet Microfluidic Systems
C. Huang*, H. Zhang*, S. -I. Han, and A. Han (*these authors contributed equally),
Analytical Chemistry, Vol. 93 (24), 8622-8630 (2021) - In-droplet Cell Separation based on Bipolar Dielectrophoretic Response to Facilitate Cellular Droplet Assays
S. Han*, C. Huang*, and A. Han (*these authors contributed equally),
Lab on a Chip, Vol. 20, pp. 3832-3841 (2020) - An Ultra High-Efficiency Droplet Microfluidics Platform using Automatically Synchronized Droplet Pairing and Merging
H. Zhang*, A. Guzman*, J. Wippold, Y. Li, J. Dai, C. Huang, and A. Han (*these authors contributed equally),
Lab on a Chip, Vol. 20, pp. 3948-3959(2020) - In-Droplet Cell Concentration using Dielectrophoresis
S. Han, H. Kim, and A. Han,
Biosensors and Bioelectronics, Vol. 97, pp. 41-45 (2017)